DNA

Part:BBa_K2637056:Design

Designed by: Yiran Liu   Group: iGEM18_Tianjin   (2018-10-05)


Gal80 donor DNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1012
    Illegal XhoI site found at 1904
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use PCR to make donor DNA from wild yeast.

Source

Saccharomyces cerevisiae

References